This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Quality Control Testing:
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of RP6-213H19.1 transfected lysate using rabbit polyclonal anti-RP6-213H19.1 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-RP6-213H19.1.
Antibody pair set content: 1. Antibody pair for IP: rabbit polyclonal anti-RP6-213H19.1 (300 ul) 2. Antibody pair for WB: mouse purified polyclonal anti-RP6-213H19.1 (50 ug)
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
The product of this gene is a member of the GCK group III family of kinases, which are a subset of the Ste20-like kinases. The encoded protein contains an amino-terminal kinase domain, and a carboxy-terminal regulatory domain that mediates homodimerization. The protein kinase localizes to the Golgi apparatus and is specifically activated by binding to the Golgi matrix protein GM130. It is also cleaved by caspase-3 in vitro, and may function in the apoptotic pathway. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq
Mst3 and SOK1-related kinase,STE20-like kinase MST4,mammalian Ste20-like protein kinase 4,mammalian sterile 20-like 4,serine/threonine protein kinase MASK