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GHRL 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00051738-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-GHRL full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 12.98
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-GHRL antibody (H00051738-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00051738-T01
    GHRL transfected lysate
    Western Blot
    QC Testing of H00051738-T01
    Lane 1: GHRL transfected lysate ( 12.98 KDa).
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • GHRL
  • Gene Alias:
  • MTLRP,obestatin
  • Gene Description:
  • ghrelin/obestatin prepropeptide
  • Gene Summary:
  • This gene encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes. Alternative promoters and alternative splicing result in multiple transcript variants, some of which encode different protein isoforms and some of which do not encode a protein but may regulate the ghrelin-obestatin preproprotein expression. In addition, antisense transcripts for this gene have been identified and may also function in regulation of the ghrelin-obestatin preproprotein expression. [provided by RefSeq
  • Other Designations:
  • ghrelin, growth hormone secretagogue receptor ligand,ghrelin/obestatin,ghrelin/obestatin preprohormone,motilin-related peptide
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