GHRL monoclonal antibody (M08), clone 3B8
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of GHRL expression in transfected 293T cell line by GHRL monoclonal antibody (M08), clone 3B8.
Lane 1: GHRL transfected lysate(12.9 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GHRL is approximately 0.3ng/ml as a capture antibody.
Western Blot detection against Immunogen (38.61 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant GHRL.
Immunogen
GHRL (AAH25791.1, 1 a.a. ~ 117 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDEMEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (82); Rat (82)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (38.61 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of GHRL expression in transfected 293T cell line by GHRL monoclonal antibody (M08), clone 3B8.
Lane 1: GHRL transfected lysate(12.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GHRL is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — GHRL
Entrez GeneID
51738GeneBank Accession#
BC025791Protein Accession#
AAH25791.1Gene Name
GHRL
Gene Alias
MTLRP, obestatin
Gene Description
ghrelin/obestatin prepropeptide
Gene Ontology
HyperlinkGene Summary
This gene encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes. Alternative promoters and alternative splicing result in multiple transcript variants, some of which encode different protein isoforms and some of which do not encode a protein but may regulate the ghrelin-obestatin preproprotein expression. In addition, antisense transcripts for this gene have been identified and may also function in regulation of the ghrelin-obestatin preproprotein expression. [provided by RefSeq
Other Designations
ghrelin, growth hormone secretagogue receptor ligand|ghrelin/obestatin|ghrelin/obestatin preprohormone|motilin-related peptide
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Interactome
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Pathway
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Disease
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