POLR3K 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
POLR3K transfected lysate.
Western Blot
Lane 1: POLR3K transfected lysate ( 11.99 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-POLR3K full-length
Host
Human
Theoretical MW (kDa)
11.99
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-POLR3K antibody (H00051728-B02) by Western Blots.
SDS-PAGE Gel
POLR3K transfected lysate.
Western Blot
Lane 1: POLR3K transfected lysate ( 11.99 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — POLR3K
Entrez GeneID
51728GeneBank Accession#
NM_016310.2Protein Accession#
-Gene Name
POLR3K
Gene Alias
C11, C11-RNP3, My010, RPC10, RPC11, hRPC11
Gene Description
polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa
Omim ID
606007Gene Ontology
HyperlinkGene Summary
This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. [provided by RefSeq
Other Designations
DNA directed RNA polymerase III polypeptide K|RNA polymerase III subunit (hRPC11)|RNA polymerase III subunit CII
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Interactome
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Pathway
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