ACSL5 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ACSL5 transfected lysate.
Western Blot
Lane 1: ACSL5 transfected lysate ( 81.4 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ACSL5 full-length
Host
Human
Theoretical MW (kDa)
81.4
Interspecies Antigen Sequence
Mouse (81); Rat (81)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ACSL5 antibody (H00051703-B01) by Western Blots.
SDS-PAGE Gel
ACSL5 transfected lysate.
Western Blot
Lane 1: ACSL5 transfected lysate ( 81.4 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ACSL5
Entrez GeneID
51703GeneBank Accession#
NM_016234.3Protein Accession#
NP_057318.2Gene Name
ACSL5
Gene Alias
ACS2, ACS5, FACL5
Gene Description
acyl-CoA synthetase long-chain family member 5
Omim ID
605677Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
Other Designations
FACL5 for fatty acid coenzyme A ligase 5|OTTHUMP00000020489|OTTHUMP00000020490|fatty acid coenzyme A ligase 5|fatty-acid-Coenzyme A ligase, long-chain 5|long-chain acyl-CoA synthetase 5|long-chain fatty acid coenzyme A ligase 5
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Interactome
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