PADI3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PADI3 transfected lysate.
Western Blot
Lane 1: PADI3 transfected lysate ( 73.15 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PADI3 full-length
Host
Human
Theoretical MW (kDa)
73.15
Interspecies Antigen Sequence
Mouse (87); Rat (87)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PADI3 antibody (H00051702-B01) by Western Blots.
SDS-PAGE Gel
PADI3 transfected lysate.
Western Blot
Lane 1: PADI3 transfected lysate ( 73.15 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PADI3
Entrez GeneID
51702GeneBank Accession#
NM_016233.1Protein Accession#
NP_057317.2Gene Name
PADI3
Gene Alias
MGC126307, MGC126308, PAD3, PDI3
Gene Description
peptidyl arginine deiminase, type III
Omim ID
606755Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type III enzyme modulates hair structural proteins, such as filaggrin in the hair follicle and trichohyalin in the inner root sheath, during hair follicle formation. Together with the type I enzyme, this enzyme may also play a role in terminal differentiation of the epidermis. This gene exists in a cluster with four other paralogous genes. [provided by RefSeq
Other Designations
OTTHUMP00000002484|peptidylarginine deiminase type III|protein-arginine deiminase type III
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