DPH5 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
DPH5 transfected lysate.
Western Blot
Lane 1: DPH5 transfected lysate ( 31.46 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DPH5 full-length
Host
Human
Theoretical MW (kDa)
31.46
Interspecies Antigen Sequence
Mouse (91); Rat (91)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DPH5 antibody (H00051611-B01) by Western Blots.
SDS-PAGE Gel
DPH5 transfected lysate.
Western Blot
Lane 1: DPH5 transfected lysate ( 31.46 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DPH5
Entrez GeneID
51611GeneBank Accession#
BC053857.1Protein Accession#
NP_001070862.1Gene Name
DPH5
Gene Alias
AD-018, CGI-30, HSPC143, MGC61450, NPD015
Gene Description
DPH5 homolog (S. cerevisiae)
Omim ID
611075Gene Ontology
HyperlinkGene Summary
This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000012522|OTTHUMP00000012523|OTTHUMP00000012524|diphthamide biosynthesis methyltransferase|diphthine synthase|protein x 0011
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