DPH5 mouse monoclonal antibody (hybridoma)
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant DPH5.
Immunogen
DPH5 (NP_001070862.1, 1 a.a. ~ 285 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MLYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLTVGKEALEEFYGRKLVVADREEVEQEADNILKDADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (91); Rat (91)
Quality Control Testing
Antibody reactivity and specificity confirmed by ELISA and Western Blot.
Deliverables
Up to 5 positive hybridoma clones will be delivered to customer in the cryotube format.
Note
Customer should check the viability of the hybridomas within one month from the date of receipt. Fee-for-service of long term hybridoma storage can be performed upon customer's request.
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Applications
Western Blot (Transfected lysate)
Western Blot (Recombinant protein)
ELISA
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Gene Info — DPH5
Entrez GeneID
51611GeneBank Accession#
NM_001077394.1Protein Accession#
NP_001070862.1Gene Name
DPH5
Gene Alias
AD-018, CGI-30, HSPC143, MGC61450, NPD015
Gene Description
DPH5 homolog (S. cerevisiae)
Omim ID
611075Gene Ontology
HyperlinkGene Summary
This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000012522|OTTHUMP00000012523|OTTHUMP00000012524|diphthamide biosynthesis methyltransferase|diphthine synthase|protein x 0011
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Interactome
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