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DPH5 rabbit monoclonal antibody

  • Catalog # : H00051611-K
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against a human DPH5 peptide using ARM Technology.
  • Immunogen:
  • A synthetic peptide of human DPH5 is used for rabbit immunization.
    Customer or Abnova will decide on the preferred peptide sequence.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Purification:
  • Protein A
  • Isotype:
  • IgG
  • Quality Control Testing:
  • Antibody reactive against human DPH5 peptide by ELISA and mammalian transfected lysate by Western Blot.
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Note:
  • 1. Customer may provide cell or tissue lysate for antibody screening.
    2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request.
  • Library Construction:
  • Non-fusion antibody library from rabbit spleen (ARM Technology).
  • Expression:
  • Overexpression vector and transfection into 293H cell line.
  • Deliverable:
  • Up to three rabbit IgG clones of 1 mg each will be delivered to customer.
  • Applications
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • ELISA
  • Gene Information
  • GeneBank Accession#:
  • DPH5
  • Gene Name:
  • DPH5
  • Gene Alias:
  • AD-018,CGI-30,HSPC143,MGC61450,NPD015
  • Gene Description:
  • DPH5 homolog (S. cerevisiae)
  • Gene Summary:
  • This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000012522,OTTHUMP00000012523,OTTHUMP00000012524,diphthamide biosynthesis methyltransferase,diphthine synthase,protein x 0011
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