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ATP6V1H 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00051606-T02
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-ATP6V1H full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 55.9
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ATP6V1H antibody (H00051606-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00051606-T02
    ATP6V1H transfected lysate.
    Western Blot
    QC Testing of H00051606-T02
    Lane 1: ATP6V1H transfected lysate ( 55.90 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • ATP6V1H
  • Gene Alias:
  • CGI-11,MSTP042,NBP1,SFD,SFDalpha,SFDbeta,VMA13
  • Gene Description:
  • ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
  • Gene Summary:
  • This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the regulatory H subunit of the V1 domain which is required for catalysis of ATP but not the assembly of V-ATPase. Three alternatively spliced transcript variants encode two isoforms of the H subunit. [provided by RefSeq
  • Other Designations:
  • ATPase, H+ transporting, lysosomal 50/57kD, V1 subunit H,V-ATPase H subunit,vacuolar ATP synthase subunit H,vacuolar ATPase subunit H,vacuolar proton pump H subunit
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