ATP6V1H 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ATP6V1H full-length
Host
Human
Theoretical MW (kDa)
53.24
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ATP6V1H antibody (H00051606-B01) by Western Blots.
SDS-PAGE Gel
ATP6V1H transfected lysate.
Western Blot
Lane 1: ATP6V1H transfected lysate ( 55.9 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ATP6V1H
Entrez GeneID
51606GeneBank Accession#
NM_015941Protein Accession#
NP_057025Gene Name
ATP6V1H
Gene Alias
CGI-11, MSTP042, NBP1, SFD, SFDalpha, SFDbeta, VMA13
Gene Description
ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
Omim ID
608861Gene Ontology
HyperlinkGene Summary
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the regulatory H subunit of the V1 domain which is required for catalysis of ATP but not the assembly of V-ATPase. Three alternatively spliced transcript variants encode two isoforms of the H subunit. [provided by RefSeq
Other Designations
ATPase, H+ transporting, lysosomal 50/57kD, V1 subunit H|V-ATPase H subunit|vacuolar ATP synthase subunit H|vacuolar ATPase subunit H|vacuolar proton pump H subunit
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