HDAC7A monoclonal antibody (M01), clone 2B11-1C1
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More Files
- More Functions
Western Blot (Cell lysate)
HDAC7A monoclonal antibody (M01), clone 2B11-1C1 Western Blot analysis of HDAC7A expression in K-562 ( Cat # L009V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of HDAC7A expression in transfected 293T cell line by HDAC7A monoclonal antibody (M01), clone 2B11-1C1.
Lane 1: HDAC7A transfected lysate(29.6 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HDAC7 is 0.3 ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of HDAC7A over-expressed 293 cell line, cotransfected with HDAC7A Validated Chimera RNAi ( Cat # H00051564-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HDAC7A monoclonal antibody (M01), clone 2B11-1C1 (Cat # H00051564-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (56.1 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant HDAC7A.
Immunogen
HDAC7A (AAH06453.1, 1 a.a. ~ 276 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MGFCFFNSVAIACRQLQQQSKASKILIVDWDVHHGNGTQQTFYQDPSVLYISLHRHDDGNFFPGSGAVDEVGAGSGEGFNVNVAWAGGLDPPMGDPEYLAAFRIVVMPIAREFSPDLVLVSAGFDAAEGHPAPLGGYHVSAKCFGYMTQQLMNLAGGAVVLALEGGHDLTAICDASEACVAALLGNRVDPLSEEGWKQKPNLNAIRSLEAVIRVHSKYWGCMQRLASCPDSWVPRVPGADKEEVEAVTALASLSVGILAEDRPSEQLVEEEEPMNL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (95); Rat (93)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (56.1 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
HDAC7A monoclonal antibody (M01), clone 2B11-1C1 Western Blot analysis of HDAC7A expression in K-562 ( Cat # L009V1 ).Western Blot (Transfected lysate)
Western Blot analysis of HDAC7A expression in transfected 293T cell line by HDAC7A monoclonal antibody (M01), clone 2B11-1C1.
Lane 1: HDAC7A transfected lysate(29.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HDAC7 is 0.3 ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of HDAC7A over-expressed 293 cell line, cotransfected with HDAC7A Validated Chimera RNAi ( Cat # H00051564-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HDAC7A monoclonal antibody (M01), clone 2B11-1C1 (Cat # H00051564-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — HDAC7
Entrez GeneID
51564GeneBank Accession#
BC006453Protein Accession#
AAH06453.1Gene Name
HDAC7
Gene Alias
DKFZp586J0917, FLJ99588, HD7A, HDAC7A
Gene Description
histone deacetylase 7
Omim ID
606542Gene Ontology
HyperlinkGene Summary
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
histone deacetylase 7A
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Interactome
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Disease
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Publication Reference
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HDAC7 and FoxA1 in estrogen-mediated repression of RPRM.
Malik S, Jiang S, Garee JP, Verdin E, Lee AV, OMalley BW, Zhang M, Belaguli NS, Oesterreich S.
Molecular and Cellular Biology 2010 Jan; 30(2):399.
Application：WB-Ce, WB-Tr, Human, DO11.10, HeLa, Ly2, MCF-7, MDA-MB-231, T47D cells.
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HDAC7 and FoxA1 in estrogen-mediated repression of RPRM.
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