VPS54 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
VPS54 transfected lysate.
Western Blot
Lane 1: VPS54 transfected lysate ( 93.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-VPS54 full-length
Host
Human
Theoretical MW (kDa)
93.2
Interspecies Antigen Sequence
Mouse (89)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-VPS54 antibody (H00051542-B01) by Western Blots.
SDS-PAGE Gel
VPS54 transfected lysate.
Western Blot
Lane 1: VPS54 transfected lysate ( 93.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — VPS54
Entrez GeneID
51542GeneBank Accession#
BC041868Protein Accession#
AAH41868Gene Name
VPS54
Gene Alias
HCC8, SLP-8p, VPS54L, hVps54L
Gene Description
vacuolar protein sorting 54 homolog (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms [provided by RefSeq
Other Designations
tumor antigen SLP-8p|vacuolar protein sorting 54
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