PPHLN1 monoclonal antibody (M01), clone 4B6
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PPHLN1.
Immunogen
PPHLN1 (NP_958846.1, 168 a.a. ~ 258 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
KVLDKPSRLTEKELAEAASKWAAEKLEKSDESNLPEISEYEAGSTAPLFTDQPEEPESNTTHGIELFEDSQLTTRSKAIASKTKEIEQVRV
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (83); Rat (81)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.75 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PPHLN1 is 0.3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PPHLN1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — PPHLN1
Entrez GeneID
51535GeneBank Accession#
NM_201438Protein Accession#
NP_958846.1Gene Name
PPHLN1
Gene Alias
HSPC206, HSPC232, MGC48786
Gene Description
periphilin 1
Omim ID
608150Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
gastric cancer antigen Ga50
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Interactome
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Disease
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