PHF7 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PHF7 transfected lysate.
Western Blot
Lane 1: PHF7 transfected lysate ( 42.02 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PHF7 full-length
Host
Human
Theoretical MW (kDa)
42.02
Interspecies Antigen Sequence
Mouse (85); Rat (81)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PHF7 antibody (H00051533-B01) by Western Blots.
SDS-PAGE Gel
PHF7 transfected lysate.
Western Blot
Lane 1: PHF7 transfected lysate ( 42.02 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PHF7
Entrez GeneID
51533GeneBank Accession#
NM_016483.4Protein Accession#
NP_057567.3Gene Name
PHF7
Gene Alias
DKFZp434L1850, HSPC045, HSPC226, MGC26088, NYD-SP6
Gene Description
PHD finger protein 7
Gene Ontology
HyperlinkGene Summary
Spermatogenesis is a complex process regulated by extracellular and intracellular factors as well as cellular interactions among interstitial cells of the testis, Sertoli cells, and germ cells. In the testis, this gene is expressed in Sertoli cells but not germ cells. However, this gene is not expressed in a patient who exhibited spermatogenic arrest at the spermatocyte stage. Spermatogenic arrest is an interruption of germ cell differentiation that may result in oligospermia or azoospermia. The proteins encoded by this gene contain plant homeodomain (PHD) finger domains, also known as leukemia associated protein (LAP) domains, believed to be involved in transcriptional regulation. Thus this protein, which localizes to the nucleus of transfected cells, has been implicated in the transcriptional regulation of spermatogenesis. Two protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq
Other Designations
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