PHF7 monoclonal antibody (M11), clone 1A7
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PHF7.
Immunogen
PHF7 (NP_057567.3, 258 a.a. ~ 357 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
GRDSFEDEGRWCLILCATCGSHGTHRDCSSLRSNSKKWECEECSPAAATDYIPENSGDIPCCSSTFHPEEHFCRDNTLEENPGLSWTDWPEPSLLEKPES
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (88); Rat (77)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.52 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PHF7 is 0.1 ng/ml as a capture antibody.ELISA
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Gene Info — PHF7
Entrez GeneID
51533GeneBank Accession#
NM_016483Protein Accession#
NP_057567.3Gene Name
PHF7
Gene Alias
DKFZp434L1850, HSPC045, HSPC226, MGC26088, NYD-SP6
Gene Description
PHD finger protein 7
Gene Ontology
HyperlinkGene Summary
Spermatogenesis is a complex process regulated by extracellular and intracellular factors as well as cellular interactions among interstitial cells of the testis, Sertoli cells, and germ cells. In the testis, this gene is expressed in Sertoli cells but not germ cells. However, this gene is not expressed in a patient who exhibited spermatogenic arrest at the spermatocyte stage. Spermatogenic arrest is an interruption of germ cell differentiation that may result in oligospermia or azoospermia. The proteins encoded by this gene contain plant homeodomain (PHD) finger domains, also known as leukemia associated protein (LAP) domains, believed to be involved in transcriptional regulation. Thus this protein, which localizes to the nucleus of transfected cells, has been implicated in the transcriptional regulation of spermatogenesis. Two protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq
Other Designations
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