C14orf129 monoclonal antibody (M06), clone 2F2
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant C14orf129.
Immunogen
C14orf129 (NP_057556.2, 1 a.a. ~ 139 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
METDCNPMELSSMSGFEEGSELNGFEGTDMKDMRLEAEAVVNDVLFAVNNMFVSKSLRCADDVAYINVETKERNRYCLELTEAGLKVVGYAFDQVDDHLQTPYHETVYSLLDTLSPAYREAFGNALLQRLEALKRDGQS
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (94); Rat (96)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (42 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of C14orf129 expression in transfected 293T cell line by C14orf129 monoclonal antibody (M06), clone 2F2.
Lane 1: C14orf129 transfected lysate(15.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged C14orf129 is 0.1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to C14orf129 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — C14orf129
Entrez GeneID
51527GeneBank Accession#
NM_016472.3Protein Accession#
NP_057556.2Gene Name
C14orf129
Gene Alias
GSKIP, HSPC210, MGC4945
Gene Description
chromosome 14 open reading frame 129
Gene Ontology
HyperlinkOther Designations
GSK3beta interaction protein
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Interactome
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Publication Reference
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GSKIP protects cardiomyocytes from hypoxia/reoxygenation-induced injury by enhancing Nrf2 activation via GSK-3β inhibition.
Li Yan, Gong Cheng, Guang Yang.
Biochemical and Biophysical Research Communications 2020 Oct; 532(1):68.
Application:WB-Ce, Mouse, Mouse cardiomyocytes.
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GSKIP protects cardiomyocytes from hypoxia/reoxygenation-induced injury by enhancing Nrf2 activation via GSK-3β inhibition.
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