NCKIPSD 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
NCKIPSD transfected lysate.
Western Blot
Lane 1: NCKIPSD transfected lysate ( 78.76 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NCKIPSD full-length
Host
Human
Theoretical MW (kDa)
78.76
Interspecies Antigen Sequence
Mouse (89); Rat (89)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NCKIPSD antibody (H00051517-B01) by Western Blots.
SDS-PAGE Gel
NCKIPSD transfected lysate.
Western Blot
Lane 1: NCKIPSD transfected lysate ( 78.76 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NCKIPSD
Entrez GeneID
51517GeneBank Accession#
NM_184231.1Protein Accession#
-Gene Name
NCKIPSD
Gene Alias
AF3P21, DIP, DIP1, MGC23891, ORF1, SPIN90, WASLBP, WISH
Gene Description
NCK interacting protein with SH3 domain
Omim ID
606671Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is localized exclusively in the cell nucleus. It plays a role in signal transduction, and may function in the maintenance of sarcomeres and in the assembly of myofibrils into sarcomeres. It also plays an important role in stress fiber formation. The gene is involved in therapy-related leukemia by a chromosomal translocation t(3;11)(p21;q23) that involves this gene and the myeloid/lymphoid leukemia gene. Alternative splicing occurs in this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq
Other Designations
SH3 protein interacting with Nck, 90 kDa|dia interacting protein|diaphanous protein interacting protein
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