YTHDF2 (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human YTHDF2 full-length ORF ( NP_057342.2, 1 a.a. - 579 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MSASSLLEQRPKGQGNKVQNGSVHQKDGLNDDDFEPYLSPQARPNNAYTAMSDSYLPSYYSPSIGFSYSLGEAAWSTGGDTAMPYLTSYGQLSNGEPHFLPDAMFGQPGALGSTPFLGQHGFNFFPSGIDFSAWGNNSSQGQSTQSSGYSSNYAYAPSSLGGAMIDGQSAFANETLNKAPGMNTIDQGMAALKLGSTEVASNVPKVVGSAVGSGSITSNIVASNSLPPATIAPPKPASWADIASKPAKQQPKLKTKNGIAGSSLPPPPIKHNMDIGTWDNKGPVAKAPSQALVQNIGQPTQGSPQPVGQQANNSPPVAQASVGQQTQPLPPPPPQPAQLSVQQQAAQPTRWVAPRNRGSGFGHNGVDGNGVGQSQAGSGSTPSEPHPVLEKLRSINNYNPKDFDWNLKHGRVFIIKSYSEDDIHRSIKYNIWCSTEHGNKRLDAAYRSMNGKGPVYLLFSVNGSGHFCGVAEMKSAVDYNTCAGVWSQDKWKGRFDVRWIFVKDVPNSQLRHIRLENNENKPVTNSRDTQEVPLEKAKQVLKIIASYKHTTSIFDDFSHYEKRQEEEESVKKERQGRGK
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
88.7
Interspecies Antigen Sequence
Mouse (99); Rat (98)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — YTHDF2
Entrez GeneID
51441GeneBank Accession#
NM_016258.2Protein Accession#
NP_057342.2Gene Name
YTHDF2
Gene Alias
HGRG8, NY-REN-2
Gene Description
YTH domain family, member 2
Gene Ontology
HyperlinkGene Summary
O
Other Designations
9430020E02Rik|OTTHUMP00000003782|YTH domain family 2|high glucose-regulated protein 8|high-glucose-regulated protein 8
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Interactome
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Publication Reference
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HSP90β Impedes STUB1-Induced Ubiquitination of YTHDF2 to Drive Sorafenib Resistance in Hepatocellular Carcinoma.
Yuning Liao, Yuan Liu, Cuifu Yu, Qiucheng Lei, Ji Cheng, Weiyao Kong, Yuanhui Yu, Xuefen Zhuang, Wenshuang Sun, Shusha Yin, Gengxi Cai, Hongbiao Huang.
Advanced Science (Weinheim, Baden-Württemberg, Germany) 2023 Sep; 10(27):e2302025.
Application:WB-Re, Human, HEK293T, HepG2/3B cells.
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HSP90β Impedes STUB1-Induced Ubiquitination of YTHDF2 to Drive Sorafenib Resistance in Hepatocellular Carcinoma.
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