ANAPC5 293T Cell Transient Overexpression Lysate(Denatured)

Catalog # H00051433-T01

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Size:100 uL
Price: USD $ 247.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
SDS-PAGE Gel
QC Test

SDS-PAGE Gel

ANAPC5 transfected lysate.

Western Blot
QC Test

Western Blot

Lane 1: ANAPC5 transfected lysate ( 85.1 KDa)
Lane 2: Non-transfected lysate.

  • Specification

    Transfected Cell Line

    293T

    Plasmid

    pCMV-ANAPC5 full-length

    Host

    Human

    Theoretical MW (kDa)

    83.16

    Interspecies Antigen Sequence

    Mouse (94); Rat (93)

    Quality Control Testing

    Transient overexpression cell lysate was tested with Anti-ANAPC5 antibody (H00051433-B01) by Western Blots.

    SDS-PAGE Gel

    ANAPC5 transfected lysate.

    Western Blot

    Lane 1: ANAPC5 transfected lysate ( 85.1 KDa)
    Lane 2: Non-transfected lysate.

    Storage Buffer

    1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

    Storage Instruction

    Store at -80°C. Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot

  • Gene Info — ANAPC5

    Entrez GeneID

    51433

    GeneBank Accession#

    NM_016237

    Protein Accession#

    NP_057321

    Gene Name

    ANAPC5

    Gene Alias

    APC5

    Gene Description

    anaphase promoting complex subunit 5

    Omim ID

    606948

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. [provided by RefSeq

    Other Designations

    anaphase-promoting complex subunit 5|cyclosome subunit 5

  • Interactome
  • Pathway
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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