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ANAPC5 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00051433-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-ANAPC5 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 83.16
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ANAPC5 antibody (H00051433-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00051433-T01
    ANAPC5 transfected lysate.
    Western Blot
    QC Testing of H00051433-T01
    Lane 1: ANAPC5 transfected lysate ( 85.1 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • ANAPC5
  • Gene Alias:
  • APC5
  • Gene Description:
  • anaphase promoting complex subunit 5
  • Gene Summary:
  • This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. [provided by RefSeq
  • Other Designations:
  • anaphase-promoting complex subunit 5,cyclosome subunit 5
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