ANAPC5 (Human) IP-WB Antibody Pair
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More Files
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Immunoprecipitation of ANAPC5 transfected lysate using rabbit polyclonal anti-ANAPC5 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-ANAPC5.
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (94); Rat (93)
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of ANAPC5 transfected lysate using rabbit polyclonal anti-ANAPC5 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-ANAPC5.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-ANAPC5 (300 ul)
2. Antibody pair for WB: mouse purified polyclonal anti-ANAPC5 (50 ug)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — ANAPC5
Entrez GeneID
51433Gene Name
ANAPC5
Gene Alias
APC5
Gene Description
anaphase promoting complex subunit 5
Omim ID
606948Gene Ontology
HyperlinkGene Summary
This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. [provided by RefSeq
Other Designations
anaphase-promoting complex subunit 5|cyclosome subunit 5
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Interactome
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Pathway
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