POLK monoclonal antibody (M01), clone 6F2
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More Files
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Western Blot (Cell lysate)
POLK monoclonal antibody (M01), clone 6F2 Western Blot analysis of POLK expression in Hela S3 NE ( Cat # L013V3 ).
Western Blot (Transfected lysate)
Western Blot analysis of POLK expression in transfected 293T cell line by POLK monoclonal antibody (M01), clone 6F2.
Lane 1: POLK transfected lysate(54.1 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to POLK on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged POLK is approximately 0.3ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of POLK over-expressed 293 cell line, cotransfected with POLK Validated Chimera RNAi ( Cat # H00051426-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with POLK monoclonal antibody (M01), clone 6F2 (Cat # H00051426-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunofluorescence
Immunofluorescence of monoclonal antibody to POLK on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (77.66 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant POLK.
Immunogen
POLK (AAH50718, 1 a.a. ~ 472 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MDSTKEKCDSYKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDRFAMELEQSRNLSNTIVHIDMDAFYAAVEMRDNPELKDKPIAVGSMSMLSTSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILADYDPNFMAMSLDEAYLNITKHLEERQNWPEDKRRYFIKMGSSVENDNPGKEVNKLSEHERSISPLLFEESPSDVQPPGDPFQVNFEEQNNPQILQNSVVFGTSAQEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPNRQAVMDFIKDLPIRKVSGIGKVTEKMLKALGIITCTELYQQRALLSLLFSETSWHYFLHISLGLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLCQELCSELAQDLQKERLKVLYFDMVSLVFKFFNSKMLP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (90); Rat (88)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (77.66 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
POLK monoclonal antibody (M01), clone 6F2 Western Blot analysis of POLK expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Transfected lysate)
Western Blot analysis of POLK expression in transfected 293T cell line by POLK monoclonal antibody (M01), clone 6F2.
Lane 1: POLK transfected lysate(54.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to POLK on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged POLK is approximately 0.3ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of POLK over-expressed 293 cell line, cotransfected with POLK Validated Chimera RNAi ( Cat # H00051426-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with POLK monoclonal antibody (M01), clone 6F2 (Cat # H00051426-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to POLK on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — POLK
Entrez GeneID
51426GeneBank Accession#
BC050718Protein Accession#
AAH50718Gene Name
POLK
Gene Alias
DINB1, DINP, POLQ
Gene Description
polymerase (DNA directed) kappa
Omim ID
605650Gene Ontology
HyperlinkOther Designations
DINB protein|DNA polymerase kappa|other name:POLK|polymerase (DNA-directed) kappa
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Interactome
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Disease
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Publication Reference
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Both High-fidelity Replicative and Low-fidelity Y-family Polymerases are Involved in DNA Rereplication.
Sekimoto T, Oda T, Kurashima K, Hanaoka F, Yamashita T.
Molecular and Cellular Biology 2015 Feb; 35(4):699.
Application：IF, WB-Ce, Human, U2OS, HCT116 cells.
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Both High-fidelity Replicative and Low-fidelity Y-family Polymerases are Involved in DNA Rereplication.
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