PRKAG2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PRKAG2 full-length
Host
Human
Theoretical MW (kDa)
37.5
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PRKAG2 antibody (H00051422-D01P) by Western Blots.
SDS-PAGE Gel
PRKAG2 transfected lysate.
Western Blot
Lane 1: PRKAG2 transfected lysate ( 37.50 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PRKAG2
Entrez GeneID
51422GeneBank Accession#
NM_024429Protein Accession#
NP_077747.1Gene Name
PRKAG2
Gene Alias
AAKG, AAKG2, CMH6, H91620p, WPWS
Gene Description
protein kinase, AMP-activated, gamma 2 non-catalytic subunit
Gene Ontology
HyperlinkGene Summary
AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family and encodes a protein with four cystathionine beta-synthase domains. Mutations in this gene have been associated with ventricular pre-excitation (Wolff-Parkinson-White syndrome), progressive conduction system disease and cardiac hypertrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
AMP-activated protein kinase gamma2 subunit|AMPK gamma2
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Interactome
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Disease
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