CDC40 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CDC40 transfected lysate.
Western Blot
Lane 1: CDC40 transfected lysate ( 65.5 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CDC40 full-length
Host
Human
Theoretical MW (kDa)
65.5
Interspecies Antigen Sequence
Mouse (98); Rat (99)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CDC40 antibody (H00051362-B01) by Western Blots.
SDS-PAGE Gel
CDC40 transfected lysate.
Western Blot
Lane 1: CDC40 transfected lysate ( 65.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CDC40
Entrez GeneID
51362GeneBank Accession#
NM_015891Protein Accession#
NP_056975Gene Name
CDC40
Gene Alias
EHB3, FLJ10564, MGC102802, PRP17, PRPF17
Gene Description
cell division cycle 40 homolog (S. cerevisiae)
Omim ID
605585Gene Ontology
HyperlinkGene Summary
Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp17 protein, which functions in two different cellular processes: pre-mRNA splicing and cell cycle progression. It suggests that this protein may play a role in cell cycle progression. [provided by RefSeq
Other Designations
EH-binding protein 3|OTTHUMP00000016997|cell division cycle 40 homolog|pre-mRNA splicing factor 17
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Interactome
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Disease
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