MARCH2 monoclonal antibody (M04), clone 7F3
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant MARCH2.
Immunogen
MARCH2 (NP_001005416.1, 1 a.a. ~ 176 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MTTGDCCHLPGSLCDCSGSPAFSKVVEATGLGPPQYVAQVTSRDGRLLSTVIRALDTPSDGPFCRICHEGANGECLLSPCGCTGTLGAVHKSCLEKWLSSSNTSYCELCHTEFAVEKRPRPLTEVSFRYHCQLYSEWRKTNQKVRLKIREADSPEGPQHSPLAAGLLKKVAEETPV
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (95); Rat (97)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (45.5 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of 2-Mar expression in transfected 293T cell line by MARCH2 monoclonal antibody (M04), clone 7F3.
Lane 1: 2-Mar transfected lysate(19.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged 2-Mar is 1 ng/ml as a capture antibody.ELISA
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Gene Info — MARCH2
Entrez GeneID
51257GeneBank Accession#
NM_001005416.1Protein Accession#
NP_001005416.1Gene Name
MARCH2
Gene Alias
HSPC240, MARCH-II, RNF172
Gene Description
membrane-associated ring finger (C3HC4) 2
Gene Ontology
HyperlinkOther Designations
membrane-associated RING-CH protein II
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