RNF181 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RNF181 transfected lysate.
Western Blot
Lane 1: RNF181 transfected lysate ( 16.94 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RNF181 full-length
Host
Human
Theoretical MW (kDa)
16.94
Interspecies Antigen Sequence
Mouse (78); Rat (81)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RNF181 antibody (H00051255-B01) by Western Blots.
SDS-PAGE Gel
RNF181 transfected lysate.
Western Blot
Lane 1: RNF181 transfected lysate ( 16.94 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RNF181
Entrez GeneID
51255GeneBank Accession#
NM_016494.3Protein Accession#
NP_057578.1Gene Name
RNF181
Gene Alias
HSPC238
Gene Description
ring finger protein 181
Gene Ontology
HyperlinkGene Summary
RNF181 binds the integrin alpha-IIb (ITGA2B; MIM 607759)/beta-3 (ITGB3; MIM 173470) complex and has E3 ubiquitin ligase activity (Brophy et al., 2008 [PubMed 18331836]).[supplied by OMIM
Other Designations
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