VRK3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
VRK3 transfected lysate.
Western Blot
Lane 1: VRK3 transfected lysate ( 45.43 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-VRK3 full-length
Host
Human
Theoretical MW (kDa)
45.43
Interspecies Antigen Sequence
Mouse (72)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-VRK3 antibody (H00051231-B01) by Western Blots.
SDS-PAGE Gel
VRK3 transfected lysate.
Western Blot
Lane 1: VRK3 transfected lysate ( 45.43 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — VRK3
Entrez GeneID
51231GeneBank Accession#
BC023556.2Protein Accession#
AAH23556.1Gene Name
VRK3
Gene Alias
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Gene Description
vaccinia related kinase 3
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. In both human and mouse, this gene has substitutions at several residues within the ATP binding motifs that in other kinases have been shown to be required for catalysis. In vitro assays indicate the protein lacks phosphorylation activity. The protein, however, likely retains its substrate binding capability. This gene is widely expressed in human tissues and its protein localizes to the nucleus. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq
Other Designations
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Interactome
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Disease
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