HERC5 (Human) Recombinant Protein (Q01)
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More Files
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Specification
Product Description
Human HERC5 partial ORF ( NP_057407, 915 a.a. - 1024 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
YDWKTFEKNARYEPGYNSSHPTIVMFWKAFHKLTLEEKKKFLVFLTGTDRLQMKDLNNMKITFCCPESWNERDPIRALTCFSVLFLPKYSTMETVEEALQEAINNNRGFG
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
37.84
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — HERC5
Entrez GeneID
51191GeneBank Accession#
NM_016323Protein Accession#
NP_057407Gene Name
HERC5
Gene Alias
CEB1, CEBP1
Gene Description
hect domain and RLD 5
Omim ID
608242Gene Ontology
HyperlinkGene Summary
This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4. [provided by RefSeq
Other Designations
HECT E3 ubiquitin ligase|HECT domain and RCC1-like domain-containing protein 5|cyclin-E binding protein 1
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