DBR1 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DBR1 full-length
Host
Human
Theoretical MW (kDa)
59.95
Interspecies Antigen Sequence
Mouse (85); Rat (85)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DBR1 antibody (H00051163-B01) by Western Blots.
SDS-PAGE Gel
DBR1 transfected lysate.
Western Blot
Lane 1: DBR1 transfected lysate ( 59.95 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DBR1
Entrez GeneID
51163GeneBank Accession#
NM_016216.2Protein Accession#
NP_057300.2Gene Name
DBR1
Gene Alias
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Gene Description
debranching enzyme homolog 1 (S. cerevisiae)
Omim ID
607024Gene Ontology
HyperlinkGene Summary
The RNA lariat debranching enzyme, or DBR1, specifically hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules.[supplied by OMIM
Other Designations
RNA lariat debranching enzyme|debranching enzyme homolog 1
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