RNF12 (Human) IP-WB Antibody Pair
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (85); Rat (86)
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of RNF12 transfected lysate using mouse monoclonal anti-RNF12 and Protein A Magnetic Bead (U0007), and immunoblotted with rabbit polyclonal anti-RNF12.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: mouse monoclonal anti-RNF12 (300 ug)
2. Antibody pair for WB: rabbit polyclonal anti-RNF12 (50 ul)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — RNF12
Entrez GeneID
51132Gene Name
RNF12
Gene Alias
MGC15161, NY-REN-43, RLIM
Gene Description
ring finger protein 12
Omim ID
300379Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
LIM domain interacting ring finger protein|OTTHUMP00000023574|OTTHUMP00000023575|ring zinc finger LIM domain binding protein|ring zinc finger protein NY-REN-43antigen
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