RNF12 MaxPab rabbit polyclonal antibody (D01)
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of RNF12 expression in transfected 293T cell line (H00051132-T02) by RNF12 MaxPab polyclonal antibody.
Lane 1: RNF12 transfected lysate(68.50 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of RNF12 transfected lysate using anti-RNF12 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead, and immunoblotted with RNF12 purified MaxPab mouse polyclonal antibody (B01P) (H00051132-B01P).
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Specification
Product Description
Rabbit polyclonal antibody raised against a full-length human RNF12 protein.
Immunogen
RNF12 (NP_057204.2, 1 a.a. ~ 624 a.a) full-length human protein.
Sequence
MENSDSNDKGSGDQSAAQRRSQMDRLDREEAFYQFVNNLSEEDYRLMRDNNLLGTPGESTEEELLRRLQQIKEGPPPQNSDENRGGDSSDDVSNGDSIIDWLNSVRQTGNTTRSGQRGNQSWRAVSRTNPNSGDFRFSLEINVNRNNGSQNSENENEPSARRSSGENVENNSQRQVENPRSESTSARPSRSERNSTEALTEVPPTRGQRRARSRSPDHRRTRARAERSRSPLHPMSEIPRRSHHSISSQTFEHPLVNETEGSSRTRHHVTLRQQISGPELLSRGLFAASGTRNASQGAGSSDTAASGESTGSGQRPPTIVLDLQVRRVRPGEYRQRDSIASRTRSRSQTPNNTVTYESERGGFRRTFSRSERAGVRTYVSTIRIPIRRILNTGLSETTSVAIQTMLRQIMTGFGELSYFMYSDSDSEPTGSVSNRNMERAESRSGRGGSGGGSSSGSSSSSSSSSSSSSSSSSSSSPSSSSGGESSETSSDLFEGSNEGSSSSGSSGARREGRHRAPVTFDESGSLPFLSLAQFFLLNEDDDDQPRGLTKEQIDNLAMRSFGENDALKTCSVCITEYTEGNKLRKLPCSHEYHVHCIDRWLSENSTCPICRRAVLASGNRESVV
Host
Rabbit
Reactivity
Human
Interspecies Antigen Sequence
Mouse (85); Rat (86)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
No additive
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of RNF12 expression in transfected 293T cell line (H00051132-T02) by RNF12 MaxPab polyclonal antibody.
Lane 1: RNF12 transfected lysate(68.50 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of RNF12 transfected lysate using anti-RNF12 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead, and immunoblotted with RNF12 purified MaxPab mouse polyclonal antibody (B01P) (H00051132-B01P). -
Gene Info — RNF12
Entrez GeneID
51132GeneBank Accession#
NM_016120.2Protein Accession#
NP_057204.2Gene Name
RNF12
Gene Alias
MGC15161, NY-REN-43, RLIM
Gene Description
ring finger protein 12
Omim ID
300379Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
LIM domain interacting ring finger protein|OTTHUMP00000023574|OTTHUMP00000023575|ring zinc finger LIM domain binding protein|ring zinc finger protein NY-REN-43antigen
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Interactome
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