SH3GLB1 monoclonal antibody (M01), clone 1B3-A5
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant SH3GLB1.
Immunogen
SH3GLB1 (AAH07455, 1 a.a. ~ 365 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MNIMDFNVKKLAADAGTFLSRAVQFTEEKLGQAEKTELDAHLENLLSKAECTKIWTEKIMKQTEVLLQPNPNARIEEFVYEKLDRKAPSRINNPELLGQYMIDAGTEFGPGTAYGNALIKCGETQKRIGTADRELIQTSALNFLTPLRNFIEGDYKTIAKERKLLQNKRLDLDAAKTRLKKAKAAETRNSSEQELRITQSEFDRQAEITRLLLEGISSTHAHHLRCLNDFVEAQMTYYAQCYQYMLDLQKQLGSFPSNYLSNNNQTSVTPVPSVLPNAIGSSAMASTSGLVITSPSNLSDLKECSGSRKARVLYDYDAANSTELSLLADEVITVFSVVGMDSDWLMGERGNQKGKVPITYLELLN
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (90); Rat (94)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (65.89 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
SH3GLB1 monoclonal antibody (M01), clone 1B3-A5 Western Blot analysis of SH3GLB1 expression in K-562 ( Cat # L009V1 ).Western Blot (Transfected lysate)
Western Blot analysis of SH3GLB1 expression in transfected 293T cell line by SH3GLB1 monoclonal antibody (M01), clone 1B3-A5.
Lane 1: SH3GLB1 transfected lysate(40.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to SH3GLB1 on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SH3GLB1 is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — SH3GLB1
Entrez GeneID
51100GeneBank Accession#
BC007455Protein Accession#
AAH07455Gene Name
SH3GLB1
Gene Alias
Bif-1, CGI-61, KIAA0491, dJ612B15.2
Gene Description
SH3-domain GRB2-like endophilin B1
Omim ID
609287Gene Ontology
HyperlinkOther Designations
Bax-interacting factor 1|OTTHUMP00000011912|SH3-containing protein SH3GLB1|endophilin B1
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Interactome
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Pathway
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Disease
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