TRNT1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
TRNT1 transfected lysate.
Western Blot
Lane 1: TRNT1 transfected lysate ( 44.66 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TRNT1 full-length
Host
Human
Theoretical MW (kDa)
44.66
Interspecies Antigen Sequence
Mouse (94)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TRNT1 antibody (H00051095-B01) by Western Blots.
SDS-PAGE Gel
TRNT1 transfected lysate.
Western Blot
Lane 1: TRNT1 transfected lysate ( 44.66 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TRNT1
Entrez GeneID
51095GeneBank Accession#
NM_016000.2Protein Accession#
NP_057084.1Gene Name
TRNT1
Gene Alias
CCA1, CGI-47, MtCCA
Gene Description
tRNA nucleotidyl transferase, CCA-adding, 1
Gene Ontology
HyperlinkGene Summary
The CCA-adding enzyme TRNT1 (EC 2.7.7.25) is an essential enzyme that catalyzes the addition of the CCA terminus to the 3-prime end of tRNA precursors. This reaction is a fundamental prerequisite for mature tRNAs to become aminoacylated and to participate in protein biosynthesis (Lizano et al., 2007 [PubMed 17204286]).[supplied by OMIM
Other Designations
mitochondrial CCA-adding tRNA-nucleotidyltransferase
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