TRNT1 monoclonal antibody (M01), clone 1G11
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of TRNT1 expression in transfected 293T cell line by TRNT1 monoclonal antibody (M01), clone 1G11.
Lane 1: TRNT1 transfected lysate (Predicted MW: 46.4 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TRNT1 is 0.3 ng/ml as a capture antibody.
Western Blot detection against Immunogen (70.29 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant TRNT1.
Immunogen
TRNT1 (AAH12537, 1 a.a. ~ 405 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MKLQSPEFQSLFTEGLKSLTELFVKENHELRIAGGAVRDLLNGVKPQDIDFATTATPTQMKEMFQSAGIRMINNRGEKHGTITARLHEENFEITTLRIDVTTDGRHAEVEFTTDWQKDAERRDLTINSMFLGFDGTLFDYFNGYEDLKNKKVRFVGHAKQRIQEDYLRILRYFRFYGRIVDKPGDHDPETLEAIAENAKGLAGISGERIWVELKKILVGNHVNHLIHLIYDLDVAPYIGLPANASLEEFDKVSKNVDGFSPKPVTLLASLFKVQDDVTKLDLRLKIAKEEKNLGLFIVKNRKDLIKATDSSDPLKPYQDFIIDSREPDATTRVCELLKYQGEHCLLKEMQQWSIPPFPVSGHDIRKVGISSGKEIGALLQQLREQWKKSGYQMEKDELLSYIKKT
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (94)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (70.29 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of TRNT1 expression in transfected 293T cell line by TRNT1 monoclonal antibody (M01), clone 1G11.
Lane 1: TRNT1 transfected lysate (Predicted MW: 46.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TRNT1 is 0.3 ng/ml as a capture antibody.ELISA
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Gene Info — TRNT1
Entrez GeneID
51095GeneBank Accession#
BC012537Protein Accession#
AAH12537Gene Name
TRNT1
Gene Alias
CCA1, CGI-47, MtCCA
Gene Description
tRNA nucleotidyl transferase, CCA-adding, 1
Gene Ontology
HyperlinkGene Summary
The CCA-adding enzyme TRNT1 (EC 2.7.7.25) is an essential enzyme that catalyzes the addition of the CCA terminus to the 3-prime end of tRNA precursors. This reaction is a fundamental prerequisite for mature tRNAs to become aminoacylated and to participate in protein biosynthesis (Lizano et al., 2007 [PubMed 17204286]).[supplied by OMIM
Other Designations
mitochondrial CCA-adding tRNA-nucleotidyltransferase
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