MRPS7 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MRPS7 transfected lysate.
Western Blot
Lane 1: MRPS7 transfected lysate ( 26.73 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MRPS7 full-length
Host
Human
Theoretical MW (kDa)
26.73
Interspecies Antigen Sequence
Mouse (84); Rat (84)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MRPS7 antibody (H00051081-B01) by Western Blots.
SDS-PAGE Gel
MRPS7 transfected lysate.
Western Blot
Lane 1: MRPS7 transfected lysate ( 26.73 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MRPS7
Entrez GeneID
51081GeneBank Accession#
NM_015971.2Protein Accession#
NP_057055.1Gene Name
MRPS7
Gene Alias
MRP-S, MRP-S7, RP-S7, RPMS7, S7mt, bMRP27a
Gene Description
mitochondrial ribosomal protein S7
Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. In the prokaryotic ribosome, the comparable protein is thought to play an essential role in organizing the 3' domain of the 16 S rRNA in the vicinity of the P- and A-sites. Pseudogenes corresponding to this gene are found on chromosomes 8p and 12p. [provided by RefSeq
Other Designations
30S ribosomal protein S7 homolog
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