MRPL2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MRPL2 transfected lysate.
Western Blot
Lane 1: MRPL2 transfected lysate ( 33.3 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MRPL2 full-length
Host
Human
Theoretical MW (kDa)
33.3
Interspecies Antigen Sequence
Mouse (83); Rat (83)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MRPL2 antibody (H00051069-B01P) by Western Blots.
SDS-PAGE Gel
MRPL2 transfected lysate.
Western Blot
Lane 1: MRPL2 transfected lysate ( 33.3 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MRPL2
Entrez GeneID
51069GeneBank Accession#
NM_015950Protein Accession#
NP_057034Gene Name
MRPL2
Gene Alias
CGI-22, MRP-L14, RPML14
Gene Description
mitochondrial ribosomal protein L2
Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 12q. [provided by RefSeq
Other Designations
OTTHUMP00000016419
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