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DHH 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00050846-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-DHH full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 43.67
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-DHH antibody (H00050846-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00050846-T01
    DHH transfected lysate.
    Western Blot
    QC Testing of H00050846-T01
    Lane 1: DHH transfected lysate ( 43.67 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • DHH
  • Gene Alias:
  • HHG-3,MGC35145
  • Gene Description:
  • desert hedgehog homolog (Drosophila)
  • Gene Summary:
  • This gene encodes a member of the Hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development. [provided by RefSeq
  • Other Designations:
  • desert hedgehog
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