DHH 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
DHH transfected lysate.
Western Blot
Lane 1: DHH transfected lysate ( 43.67 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DHH full-length
Host
Human
Theoretical MW (kDa)
43.67
Interspecies Antigen Sequence
Mouse (96); Rat (96)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DHH antibody (H00050846-B01) by Western Blots.
SDS-PAGE Gel
DHH transfected lysate.
Western Blot
Lane 1: DHH transfected lysate ( 43.67 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DHH
Entrez GeneID
50846GeneBank Accession#
NM_021044.2Protein Accession#
NP_066382.1Gene Name
DHH
Gene Alias
HHG-3, MGC35145
Gene Description
desert hedgehog homolog (Drosophila)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development. [provided by RefSeq
Other Designations
desert hedgehog
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Interactome
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Pathway
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