NSDHL 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NSDHL transfected lysate.
Western Blot
Lane 1: NSDHL transfected lysate ( 41.90 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NSDHL full-length
Host
Human
Theoretical MW (kDa)
41.9
Interspecies Antigen Sequence
Mouse (82); Rat (82)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NSDHL antibody (H00050814-D01P) by Western Blots.
SDS-PAGE Gel
NSDHL transfected lysate.
Western Blot
Lane 1: NSDHL transfected lysate ( 41.90 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NSDHL
Entrez GeneID
50814GeneBank Accession#
NM_015922.1Protein Accession#
NP_057006.1Gene Name
NSDHL
Gene Alias
H105E3, SDR31E1, XAP104
Gene Description
NAD(P) dependent steroid dehydrogenase-like
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000025902|short chain dehydrogenase/reductase family 31E, member 1|sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating
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Interactome
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