DDEF1 monoclonal antibody (M01), clone 2G7
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant DDEF1.
Immunogen
DDEF1 (NP_060952, 1030 a.a. ~ 1129 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
VQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD
Host
Mouse
Reactivity
Human, Rat
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
DDEF1 monoclonal antibody (M01), clone 2G7 Western Blot analysis of DDEF1 expression in IMR-32 ( Cat # L008V1 ).Western Blot (Cell lysate)
DDEF1 monoclonal antibody (M01), clone 2G7. Western Blot analysis of DDEF1 expression in PC-12 ( Cat # L012V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DDEF1 is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to DDEF1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — ASAP1
Entrez GeneID
50807GeneBank Accession#
NM_018482Protein Accession#
NP_060952Gene Name
ASAP1
Gene Alias
AMAP1, CENTB4, DDEF1, KIAA1249, PAG2, PAP, ZG14P
Gene Description
ArfGAP with SH3 domain, ankyrin repeat and PH domain 1
Omim ID
605953Gene Ontology
HyperlinkGene Summary
O
Other Designations
centaurin, beta 4|development and differentiation enhancing factor 1
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Interactome
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Pathway
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Publication Reference
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The small molecule inhibitor NAV-2729 has a complex target profile including multiple ADP-ribosylation factor regulatory proteins.
Eric M Rosenberg Jr, Xiaoying Jian, Olivier Soubias, Hye-Young Yoon, Mukesh P Yadav, Sarah Hammoudeh, Sandeep Pallikkuth, Itoro Akpan, Pei-Wen Chen, Tapan K Maity, Lisa M Jenkins, Marielle E Yohe, R Andrew Byrd, Paul A Randazzo.
The Journal of Biological Chemistry 2023 Feb; 299(3):102992.
Application:WB-Tr, Human, U2OS cells.
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The small molecule inhibitor NAV-2729 has a complex target profile including multiple ADP-ribosylation factor regulatory proteins.
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