Western blot analysis of PIK3R4 over-expressed 293 cell line, cotransfected with PIK3R4 Validated Chimera RNAi ( Cat # H00030849-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PIK3R4 monoclonal antibody (M01) clone 1D4 (Cat # H00030849-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.