EHD2 monoclonal antibody (M01), clone 2D8
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant EHD2.
Immunogen
EHD2 (NP_055416, 365 a.a. ~ 427 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
AHDFTKFHSLKPKLLEALDEMLTHDIAKLMPLLRQEELESTEVGVQGGAFEGTHMGPFVERGP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (96); Rat (97)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (32.67 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
EHD2 monoclonal antibody (M01), clone 2D8 Western Blot analysis of EHD2 expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of EHD2 expression in transfected 293T cell line by EHD2 monoclonal antibody (M01), clone 2D8.
Lane 1: EHD2 transfected lysate(61.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EHD2 is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to EHD2 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — EHD2
Entrez GeneID
30846GeneBank Accession#
NM_014601Protein Accession#
NP_055416Gene Name
EHD2
Gene Alias
FLJ96617, PAST2
Gene Description
EH-domain containing 2
Omim ID
605890Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. [provided by RefSeq
Other Designations
EH domain containing 2
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Interactome
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Pathway
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