EHD3 monoclonal antibody (M01), clone 4B7
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant EHD3.
Immunogen
EHD3 (NP_055415, 357 a.a. ~ 406 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
KRMQDQLQAQDFSKFQPLKSKLLEVVDDMLAHDIAQLMVLVRQEESQRPI
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (31.24 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
EHD3 monoclonal antibody (M01), clone 4B7. Western Blot analysis of EHD3 expression in MCF-7 ( Cat # L046V1 ).Western Blot (Cell lysate)
EHD3 monoclonal antibody (M01), clone 4B7 Western Blot analysis of EHD3 expression in IMR-32 ( Cat # L008V1 ).Western Blot (Cell lysate)
EHD3 monoclonal antibody (M01), clone 4B7. Western Blot analysis of EHD3 expression in COLO 320 HSR ( Cat # L020V1 ).Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to EHD3 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EHD3 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to EHD3 on HeLa cell. [antibody concentration 25 ug/ml] -
Gene Info — EHD3
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Interactome
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Pathway
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Publication Reference
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The HIV-1 protein Vpr impairs phagosome maturation by controlling microtubule-dependent trafficking.
Dumas A, Le-Bury G, Marie-Anais F, Herit F, Mazzolini J, Guilbert T, Bourdoncle P, Russell DG, Benichou S, Zahraoui A, Niedergang F.
The Journal of Cell Biology 2015 Oct; 211(2):359.
Application:IF, Human, Macrophages.
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The HIV-1 protein Vpr impairs phagosome maturation by controlling microtubule-dependent trafficking.
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