EFEMP2 monoclonal antibody (M19), clone 2C8
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of EFEMP2 expression in transfected 293T cell line by EFEMP2 monoclonal antibody (M01), clone 2C8.
Lane 1: EFEMP2 transfected lysate(49.4 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of EFEMP2 transfected lysate using anti-EFEMP2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EFEMP2 MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EFEMP2 is 0.3 ng/ml as a capture antibody.
Western Blot detection against Immunogen (71.61 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant EFEMP2.
Immunogen
EFEMP2 (AAH10456.1, 26 a.a. ~ 443 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
SPQDSEEPDSYTECTDGYEWDPDSQHCRDVNECLTIPEACKGEMKCINHYGGYLCLPRSAAVINDLHGEGPPPPVPPAQHPNPCPPGYEPDDQDSCVDVDECAQALHDCRPSQDCHNLPGSYQCTCPDGYRKIGPECVDIDECRYRYCQHRCVNLPGSFRCQCEPGFQLGPNNRSCVDVNECDMGAPCEQRCFNSYGTFLCRCHQGYELHRDGFSCSDIDECSYSSYLCQYRCVNEPGRFSCHCPQGYQLLATRLCQDIDECESGAHQCSEAQTCVNFHGGYRCVDTNRCVEPYIQVSENRCLCPASNPLCREQPSSIVHRYMTITSERSVPADVFQIQATSVYPGAYNAFQIRAGNSQGDFYIRQINNVSAMLVLARPVTGPREYVLDLEMVTMNSLMSYRASSVLRLTVFVGAYTF
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (95); Rat (96)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (71.61 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of EFEMP2 expression in transfected 293T cell line by EFEMP2 monoclonal antibody (M01), clone 2C8.
Lane 1: EFEMP2 transfected lysate(49.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of EFEMP2 transfected lysate using anti-EFEMP2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EFEMP2 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EFEMP2 is 0.3 ng/ml as a capture antibody.ELISA
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Gene Info — EFEMP2
Entrez GeneID
30008GeneBank Accession#
BC010456Protein Accession#
AAH10456.1Gene Name
EFEMP2
Gene Alias
FBLN4, MBP1, UPH1
Gene Description
EGF-containing fibulin-like extracellular matrix protein 2
Gene Ontology
HyperlinkGene Summary
A large number of extracellular matrix proteins have been found to contain variations of the epidermal growth factor (EGF) domain and have been implicated in functions as diverse as blood coagulation, activation of complement and determination of cell fate during development. The protein encoded by this gene contains four EGF2 domains and six calcium-binding EGF2 domains. This gene is necessary for elastic fiber formation and connective tissue development. Defects in this gene are cause of an autosomal recessive cutis laxa syndrome. [provided by RefSeq
Other Designations
fibulin 4|fibulin-like extracellular matrix protein|mutant p53 binding protein 1
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