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ACF 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00029974-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-ACF full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 65
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ACF antibody (H00029974-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00029974-T01
    A1CF transfected lysate.
    Western Blot
    QC Testing of H00029974-T01
    Lane 1: A1CF transfected lysate ( 65 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • A1CF
  • Gene Alias:
  • ACF,ACF64,ACF65,APOBEC1CF,ASP,MGC163391,RP11-564C4.2
  • Gene Description:
  • APOBEC1 complementation factor
  • Gene Summary:
  • Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Alternative splicing occurs at this locus and three full-length transcript variants, encoding three distinct isoforms, have been described. Additional splicing has been observed but the full-length nature of these variants has not been determined. [provided by RefSeq
  • Other Designations:
  • APOBEC-1 stimulating protein,OTTHUMP00000019611,OTTHUMP00000019612,OTTHUMP00000019614,OTTHUMP00000019615,OTTHUMP00000061209,apo-B RNA editing protein,apobec-1 complementation factor,apobec-1 complementation factor (ACF) (ASP)
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