ANAPC4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ANAPC4 transfected lysate.
Western Blot
Lane 1: ANAPC4 transfected lysate ( 88.99 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ANAPC4 full-length
Host
Human
Theoretical MW (kDa)
88.99
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ANAPC4 antibody (H00029945-B01) by Western Blots.
SDS-PAGE Gel
ANAPC4 transfected lysate.
Western Blot
Lane 1: ANAPC4 transfected lysate ( 88.99 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ANAPC4
Entrez GeneID
29945GeneBank Accession#
NM_013367Protein Accession#
NP_037499Gene Name
ANAPC4
Gene Alias
APC4
Gene Description
anaphase promoting complex subunit 4
Omim ID
606947Gene Ontology
HyperlinkGene Summary
A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known. [provided by RefSeq
Other Designations
anaphase-promoting complex subunit 4
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Interactome
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Pathway
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