DSE monoclonal antibody (M03), clone 6D4
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant DSE.
Immunogen
DSE (NP_037484, 574 a.a. ~ 673 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
LGEESPLETAASFFHNVDVPFEETVVDGVHGAFIRQRDGLYKMYWMDDTGYSEKATFASVTYPRGYPYNGTNYVNVTMHLRSPITRAAYLFIGPSIDVQS
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (96); Rat (99)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of DSE expression in transfected 293T cell line by DSE monoclonal antibody (M03), clone 6D4.
Lane 1: DSE transfected lysate (Predicted MW: 109.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DSE is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — DSE
Entrez GeneID
29940GeneBank Accession#
NM_013352Protein Accession#
NP_037484Gene Name
DSE
Gene Alias
DSEPI, SART2
Gene Description
dermatan sulfate epimerase
Omim ID
605942Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is localized to the endoplasmic reticulum. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000017059|OTTHUMP00000040406|squamous cell carcinoma antigen recognized by T cells 2
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Interactome
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Pathway
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