NENF monoclonal antibody (M04), clone 7D10
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant NENF.
Immunogen
NENF (NP_037481.1, 1 a.a. ~ 172 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MVGPAPRRRLRPLAALALVLALAPGLPTARAGQTPRPAERGPPVRLFTEEELARYGGEEEDQPIYLAVKGVVFDVTSGKEFYGRGAPYNALTGKDSTRGVAKMSLDPADLTHDTTGLTAKELEALDEVFTKVYKAKYPIVGYTARRILNEDGSPNLDFKPEDQPHFDIKDEF
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (95); Rat (88)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (45.3 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of NENF expression in transfected 293T cell line by NENF monoclonal antibody (M04), clone 7D10.
Lane 1: NENF transfected lysate (Predicted MW: 18.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NENF is 0.1 ng/ml as a capture antibody.ELISA
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Gene Info — NENF
Entrez GeneID
29937GeneBank Accession#
NM_013349.3Protein Accession#
NP_037481.1Gene Name
NENF
Gene Alias
CIR2, NEUDESIN, SCIRP10, SPUF
Gene Description
neuron derived neurotrophic factor
Gene Ontology
HyperlinkGene Summary
This gene encodes a neurotrophic factor that may play a role in neuron differentiation and development. A pseudogene of this gene is found on chromosome 12. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000034886|SCIRP10-related protein|Spinal cord injury related protein 10|cell growth-inhibiting protein 47|secreted protein of unknown function
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