EEF2K 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
EEF2K transfected lysate.
Western Blot
Lane 1: EEF2K transfected lysate ( 79.86 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-EEF2K full-length
Host
Human
Theoretical MW (kDa)
79.86
Interspecies Antigen Sequence
Mouse (90); Rat (90)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-EEF2K antibody (H00029904-B01) by Western Blots.
SDS-PAGE Gel
EEF2K transfected lysate.
Western Blot
Lane 1: EEF2K transfected lysate ( 79.86 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — EEF2K
Entrez GeneID
29904GeneBank Accession#
BC032665.1Protein Accession#
AAH32665.1Gene Name
EEF2K
Gene Alias
HSU93850, MGC45041, eEF-2K
Gene Description
eukaryotic elongation factor-2 kinase
Omim ID
606968Gene Ontology
HyperlinkGene Summary
This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq
Other Designations
calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase|calmodulin-dependent protein kinase III|eEF-2 kinase|elongation factor-2 kinase
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Interactome
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Disease
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