ANAPC2 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ANAPC2 full-length
Host
Human
Theoretical MW (kDa)
90.53
Interspecies Antigen Sequence
Mouse (94); Rat (96)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ANAPC2 antibody (H00029882-B01) by Western Blots.
SDS-PAGE Gel
ANAPC2 transfected lysate.
Western Blot
Lane 1: ANAPC2 transfected lysate ( 90.42 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ANAPC2
Entrez GeneID
29882GeneBank Accession#
BC032503Protein Accession#
AAH32503Gene Name
ANAPC2
Gene Alias
APC2, RP11-350O14.5
Gene Description
anaphase promoting complex subunit 2
Omim ID
606946Gene Ontology
HyperlinkGene Summary
A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. [provided by RefSeq
Other Designations
OTTHUMP00000022692|anaphase-promoting complex subunit 2
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