ALG5 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ALG5 full-length
Host
Human
Theoretical MW (kDa)
35.75
Interspecies Antigen Sequence
Mouse (91); Rat (92)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ALG5 antibody (H00029880-B01) by Western Blots.
SDS-PAGE Gel
ALG5 transfected lysate.
Western Blot
Lane 1: ALG5 transfected lysate ( 35.75 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ALG5
Entrez GeneID
29880GeneBank Accession#
NM_013338.3Protein Accession#
NP_037470.1Gene Name
ALG5
Gene Alias
RP11-421P11.2, bA421P11.2
Gene Description
asparagine-linked glycosylation 5, dolichyl-phosphate beta-glucosyltransferase homolog (S. cerevisiae)
Omim ID
604565Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the glycosyltransferase 2 family. The encoded protein participates in glucosylation of the oligomannose core in N-linked glycosylation of proteins. The addition of glucose residues to the oligomannose core is necessary to ensure substrate recognition, and therefore, effectual transfer of the oligomannose core to the nascent glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
Alg5, S. cerevisiae, homolog of|OTTHUMP00000042273|asparagine-linked glycosylation 5 homolog (S. cerevisiae, dolichyl-phosphate beta-glucosyltransferase)|asparagine-linked glycosylation 5 homolog (yeast, dolichyl-phosphate beta-glucosyltransferase)|dolich
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Interactome
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